LongSAGE profiling of nine human embryonic stem cell lines

Genome Biol. 2007;8(6):R113. doi: 10.1186/gb-2007-8-6-r113.

Abstract

To facilitate discovery of novel human embryonic stem cell (ESC) transcripts, we generated 2.5 million LongSAGE tags from 9 human ESC lines. Analysis of this data revealed that ESCs express proportionately more RNA binding proteins compared with terminally differentiated cells, and identified novel ESC transcripts, at least one of which may represent a marker of the pluripotent state.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Base Sequence
  • Cell Line
  • Embryonic Stem Cells / metabolism*
  • Gene Expression Profiling*
  • Humans
  • Pluripotent Stem Cells / metabolism*
  • RNA-Binding Proteins / genetics
  • Sequence Alignment

Substances

  • RNA-Binding Proteins