Abstract
The present study used isobaric tags for relative and absolute quantitation (iTRAQ) to identify novel targets in experimental autoimmune encephalomyelitis (EAE), an animal model of multiple sclerosis. The expression of 41 proteins was significantly altered in the inflamed spinal cord. Twenty of these are implicated in EAE for the first time and many have previously been shown to play a role in antigen processing, inflammation, neuroprotection, or neurodegeneration.
Publication types
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Research Support, N.I.H., Extramural
MeSH terms
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Amino Acid Sequence
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Animals
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Blotting, Western
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Encephalomyelitis, Autoimmune, Experimental / metabolism*
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Female
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Macrophages / chemistry
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Macrophages / metabolism
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Microglia / chemistry
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Microglia / metabolism
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Molecular Sequence Data
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Multiple Sclerosis / metabolism*
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Muscle Proteins / analysis
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Muscle Proteins / metabolism
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Nerve Tissue Proteins / analysis*
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Nerve Tissue Proteins / metabolism
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Proteasome Endopeptidase Complex / analysis
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Proteasome Endopeptidase Complex / metabolism
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Proteomics*
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Rats
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Rats, Inbred Lew
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Spinal Cord / chemistry*
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Spinal Cord / metabolism
Substances
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Muscle Proteins
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Nerve Tissue Proteins
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PSME1 protein, human
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Proteasome Endopeptidase Complex