Impact of hepatitis C virus (HCV) genotypes on quantification of HCV RNA in serum by COBAS AmpliPrep/COBAS TaqMan HCV test, Abbott HCV realtime assay [corrected] and VERSANT HCV RNA assay

Edouard Tuaillon; Anne-Marie Mondain; Laure Ottomani; Laurent Roudière; Pascal Perney; Marie-Christine Picot; Fabienne Séguret; François Blanc; Dominique Larrey; Philippe Van de Perre; Jacques Ducos

doi:10.1128/JCM.00111-07

Impact of hepatitis C virus (HCV) genotypes on quantification of HCV RNA in serum by COBAS AmpliPrep/COBAS TaqMan HCV test, Abbott HCV realtime assay [corrected] and VERSANT HCV RNA assay

J Clin Microbiol. 2007 Sep;45(9):3077-81. doi: 10.1128/JCM.00111-07. Epub 2007 Jun 20.

Authors

Edouard Tuaillon¹, Anne-Marie Mondain, Laure Ottomani, Laurent Roudière, Pascal Perney, Marie-Christine Picot, Fabienne Séguret, François Blanc, Dominique Larrey, Philippe Van de Perre, Jacques Ducos

Affiliation

¹ Unité Hépatites Virales, Laboratoire de Virologie, Hôpital Lapeyronie, av. du Doyen G. Giraud, 34295 Montpellier, France.

Abstract

The VERSANT HCV RNA 3.0 (bDNA), COBAS AmpliPrep/COBAS TaqMan HCV, and Abbott ART HCV RealTime assays were compared for hepatitis C virus RNA quantification in 158 clinical specimens (genotypes 1 to 5). RNA values differed significantly between methods (P < 0.0001), and mean titer differences ranged from 0.01 to 0.50 log(10) IU/ml depending on the genotypes.

Publication types

Comparative Study
Evaluation Study
Research Support, Non-U.S. Gov't

MeSH terms

Hepacivirus / genetics*
Hepatitis C / virology*
Humans
Molecular Diagnostic Techniques / methods*
RNA, Viral / blood*
Reagent Kits, Diagnostic
Serum / virology*
Viral Load / methods*

Substances

RNA, Viral
Reagent Kits, Diagnostic