Evaluation of methods to identify the Klebsiella pneumoniae carbapenemase in Enterobacteriaceae

J Clin Microbiol. 2007 Aug;45(8):2723-5. doi: 10.1128/JCM.00015-07. Epub 2007 Jun 20.

Abstract

The Klebsiella pneumoniae carbapenem (KPC) beta-lactamase occurs in Enterobacteriaceae and can confer resistance to all beta-lactam agents including carbapenems. The enzyme may confer low-level carbapenem resistance, and the failure of susceptibility methods to identify this resistance has been reported. Automated and nonautomated methods for carbapenem susceptibility were evaluated for identification of KPC-mediated resistance. Ertapenem was a more sensitive indicator of KPC resistance than meropenem and imipenem independently of the method used. Carbapenemase production could be confirmed with the modified Hodge test.

Publication types

  • Evaluation Study

MeSH terms

  • Anti-Bacterial Agents / pharmacology
  • Bacterial Proteins / analysis*
  • Enterobacteriaceae / enzymology*
  • Microbial Sensitivity Tests / methods*
  • beta-Lactam Resistance*
  • beta-Lactamases / analysis*
  • beta-Lactams / pharmacology

Substances

  • Anti-Bacterial Agents
  • Bacterial Proteins
  • beta-Lactams
  • beta-Lactamases
  • carbapenemase