Identification of immunogenic MHC class II Tyrosinase-derived peptides using HLA-DR1 and HLA-DR4 transgenic mice

R B V Horton; S A S Laversin; S P Reeder; R C Rees; S E B McArdle

doi:10.2174/092986607780782768

Identification of immunogenic MHC class II Tyrosinase-derived peptides using HLA-DR1 and HLA-DR4 transgenic mice

Protein Pept Lett. 2007;14(5):455-60. doi: 10.2174/092986607780782768.

Authors

R B V Horton¹, S A S Laversin, S P Reeder, R C Rees, S E B McArdle

Affiliation

¹ The School of Biomedical and Natural Science, Nottingham Trent University, Clifton Campus, Interdisciplinary Biomedical Research Centre, Clifton Lane, Clifton, Nottingham, NG11 8NS, UK.

PMID: 17584170
DOI: 10.2174/092986607780782768

Abstract

The immunogenicity of "novel" MART-1 and Tyrosinase class-II peptides was assessed in transgenic mice. Tyrosinase(141-161) peptide was found to be immunogenic and endogenously processed in the HLA-DRbeta1*0101 and HLA-DRbeta1*0401 transgenic mice with peptide specific production of IFNgamma or IL-5 respectively. The MART-1(29-43) peptide was only found immunogenic in HLA-DRbeta1*0101 mice.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Cells, Cultured
Granulocytes
HLA Antigens / immunology*
HLA-DR1 Antigen / immunology
HLA-DR4 Antigen / immunology
Isoantigens / isolation & purification*
Melanoma / immunology
Mice
Mice, Transgenic
Monophenol Monooxygenase / immunology
Monophenol Monooxygenase / metabolism*
Peptide Fragments / immunology
T-Lymphocytes / immunology*

Substances

HLA Antigens
HLA-DR1 Antigen
HLA-DR4 Antigen
HNA-4A antigen
Isoantigens
Peptide Fragments
Monophenol Monooxygenase