Purification and concentration of bacteriophage T4 using monolithic chromatographic supports

F Smrekar; M Ciringer; M Peterka; A Podgornik; A Strancar

doi:10.1016/j.jchromb.2007.05.048

Purification and concentration of bacteriophage T4 using monolithic chromatographic supports

J Chromatogr B Analyt Technol Biomed Life Sci. 2008 Jan 15;861(2):177-80. doi: 10.1016/j.jchromb.2007.05.048. Epub 2007 Jun 7.

Authors

F Smrekar¹, M Ciringer, M Peterka, A Podgornik, A Strancar

Affiliation

¹ BIA Separations d.o.o., Teslova 30, SI-1000 Ljubljana, Slovenia.

PMID: 17588505
DOI: 10.1016/j.jchromb.2007.05.048

Abstract

Phages are gaining importance due to their wide usage. In this work strong anion exchange monolithic chromatographic column was used for single step phage purification. Most of the proteins and DNA were removed and recovery of approximately 70% of infective virus was reproducibly achieved. 30 ml of phage sample was purified in around 10 min.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Anion Exchange Resins
Bacteriophage T4 / drug effects
Bacteriophage T4 / isolation & purification*
Chromatography, High Pressure Liquid
Chromatography, Ion Exchange / methods*
Hydrogen-Ion Concentration
Reproducibility of Results
Sodium Chloride / pharmacology

Substances

Anion Exchange Resins
Sodium Chloride