Abstract
Human cytomegalovirus (HCMV) has a very high infection rate in the normal population. Early diagnosis is of great value, especially in immunosuppressed individuals. HCMV phosphoprotein 65 (pp65) is the most widely used antigen for diagnosis. In this report, we prepared a monoclonal antibody against pp65, which could be used for both immunohistochemistry and Western blot diagnosis of HCMV infection.
MeSH terms
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Animals
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Antibodies, Monoclonal / biosynthesis*
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Antigens, Viral / immunology*
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Blotting, Western
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Cytomegalovirus / immunology*
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Cytomegalovirus Infections / diagnosis
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Enzyme-Linked Immunosorbent Assay
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Humans
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Hybridomas / immunology
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Immunohistochemistry
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Mice
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Mice, Inbred BALB C
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Phosphoproteins / immunology*
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Viral Matrix Proteins / immunology*
Substances
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Antibodies, Monoclonal
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Antigens, Viral
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Phosphoproteins
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Viral Matrix Proteins
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cytomegalovirus matrix protein 65kDa