Complex formation between cationic beta-1,3-glucan and hetero-sequence oligodeoxynucleotide and its delivery into macrophage-like cells to induce cytokine secretion

Org Biomol Chem. 2007 Jul 21;5(14):2219-24. doi: 10.1039/b705997c. Epub 2007 Jun 7.

Abstract

A cationic polysaccharide bearing a beta-1,3-glucan main-chain structure (CUR-N(+)) forms a complex with a hetero-sequence oligonucleotide, that is, a CpG ODN, and facilitates the transportation of the resultant complex into a murine macrophage-like cell J774.A1, which induces an efficient secretion of a cytokine (IL-12) as compared with that induced by conventional carriers such as poly(ethyleneimine) (PEI) and poly(L-lysine) (PLL).

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adjuvants, Immunologic / administration & dosage*
  • Adjuvants, Immunologic / chemistry
  • Adjuvants, Immunologic / metabolism
  • Animals
  • Cells, Cultured
  • Circular Dichroism
  • Cytokines / metabolism*
  • Drug Carriers / chemical synthesis
  • Drug Carriers / chemistry*
  • Macrophages / immunology*
  • Mice
  • Microscopy, Confocal
  • Models, Biological
  • Oligodeoxyribonucleotides / administration & dosage*
  • Oligodeoxyribonucleotides / chemistry
  • Oligodeoxyribonucleotides / metabolism
  • Polysaccharides / chemistry
  • Quaternary Ammonium Compounds / chemistry*
  • beta-Glucans / chemistry*

Substances

  • Adjuvants, Immunologic
  • CPG-oligonucleotide
  • CUR-N beta-1,3-glucan
  • Cytokines
  • Drug Carriers
  • Oligodeoxyribonucleotides
  • Polysaccharides
  • Quaternary Ammonium Compounds
  • beta-Glucans
  • curdlan
  • beta-1,3-glucan