Abstract
We have cloned and sequenced mouse brain AP47, the medium chain of the trans-Golgi network clathrin-associated protein complex AP-1. The predicted protein sequence of AP47 is closely related to rat and calf brain AP50, the corresponding medium chain of the plasma-membrane clathrin-associated protein complex AP-2. We have also identified in the yeast genome an open reading frame encoding a protein of previously unknown function. Referred to here as YAP54, its predicted protein sequence displays a striking homology to AP47. We therefore propose that Yap54 is the medium chain subunit of a putative AP-1 complex in yeast. From the analyses of the optimized sequence alignments of AP47, AP50 and Yap54p, we suggest a model for the domain organization of the medium chains.
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Adaptor Protein Complex 2*
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Adaptor Protein Complex mu Subunits*
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Adaptor Proteins, Vesicular Transport
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Amino Acid Sequence
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Animals
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Base Sequence
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Blotting, Southern
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Cattle
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Clathrin / metabolism*
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Cloning, Molecular
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DNA / genetics
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Fungal Proteins / analysis
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Fungal Proteins / chemistry
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Fungal Proteins / genetics*
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Molecular Sequence Data
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Phosphoproteins / analysis
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Phosphoproteins / chemistry
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Phosphoproteins / genetics*
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Rats
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Saccharomyces cerevisiae / chemistry
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Saccharomyces cerevisiae / genetics*
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Sequence Alignment
Substances
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Adaptor Protein Complex 2
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Adaptor Protein Complex mu Subunits
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Adaptor Proteins, Vesicular Transport
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Clathrin
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Fungal Proteins
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Phosphoproteins
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adaptor protein complex 2, mu 1 subunit
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adaptor protein complex 2, mu 2 subunit
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DNA
Associated data
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GENBANK/M60272
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GENBANK/M60273
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GENBANK/M60352
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GENBANK/M60353
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GENBANK/M60354
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GENBANK/M62419
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GENBANK/S65077
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GENBANK/S74763
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GENBANK/S74785
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GENBANK/X56257
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GENBANK/X60288