Genetic polymorphism of the adenosine A2A receptor is associated with habitual caffeine consumption

Am J Clin Nutr. 2007 Jul;86(1):240-4. doi: 10.1093/ajcn/86.1.240.

Abstract

Background: Caffeine is the most widely consumed stimulant in the world, and individual differences in response to its stimulating effects may explain some of the variability in caffeine consumption within a population.

Objective: We examined whether genetic variability in caffeine metabolism [cytochrome P450 1A2 (CYP1A2) -163A-->C] or the main target of caffeine action in the nervous system [adenosine A(2A) receptor (ADORA2A) 1083C-->T] is associated with habitual caffeine consumption.

Design: Subjects (n=2735) were participants from a study of gene-diet interactions and risk of myocardial infarction who did not have a history of hypertension. Genotype frequencies were examined among persons who were categorized according to their self-reported daily caffeine intake, as assessed with a validated food-frequency questionnaire.

Results: The ADORA2A, but not the CYP1A2, genotype was associated with different amounts of caffeine intake. Compared with persons consuming <100 mg caffeine/d, the odds ratios for having the ADORA2A TT genotype were 0.74 (95% CI: 0.53, 1.03), 0.63 (95% CI: 0.48, 0.83), and 0.57 (95% CI: 0.42, 0.77) for those consuming 100-200, >200-400, and >400 mg caffeine/d, respectively. The association was more pronounced among current smokers than among nonsmokers (P for interaction = 0.07). Persons with the ADORA2A TT genotype also were significantly more likely to consume less caffeine (ie, <100 mg/d) than were carriers of the C allele [P=0.011 (nonsmokers), P=0.008 (smokers)].

Conclusion: Our findings show that the probability of having the ADORA2A 1083TT genotype decreases as habitual caffeine consumption increases. This observation provides a biologic basis for caffeine consumption behavior and suggests that persons with this genotype may be less vulnerable to caffeine dependence.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Alcohol Drinking
  • Caffeine / administration & dosage*
  • Case-Control Studies
  • Costa Rica
  • Cytochrome P-450 CYP1A2 / genetics
  • Cytochrome P-450 CYP1A2 / metabolism
  • DNA / chemistry
  • DNA / genetics
  • Drinking Behavior / physiology*
  • Female
  • Genetic Variation
  • Genotype
  • Humans
  • Logistic Models
  • Male
  • Middle Aged
  • Polymerase Chain Reaction
  • Polymorphism, Restriction Fragment Length
  • Polymorphism, Single Nucleotide
  • Receptors, Adenosine A2 / genetics*
  • Receptors, Adenosine A2 / metabolism
  • Smoking
  • Substance-Related Disorders / genetics
  • Substance-Related Disorders / metabolism

Substances

  • Receptors, Adenosine A2
  • Caffeine
  • DNA
  • CYP1A2 protein, human
  • Cytochrome P-450 CYP1A2