Abstract
Three actinomycetes isolated from the surfaces of rocks in a medieval slate mine were examined in a polyphasic taxonomic study. Chemotaxonomic and morphological characteristics of the isolates were typical of strains of the genus Amycolatopsis. The isolates had identical 16S rRNA gene sequences and formed a distinct phyletic line towards the periphery of the Amycolatopsis mediterranei clade, being most closely related to Amycolatopsis rifamycinica. The organisms shared a wide range of genotypic and phenotypic markers that distinguished them from their closest phylogenetic neighbours. On the basis of these results, a novel species, Amycolatopsis saalfeldensis sp. nov., is proposed. The type strain is HKI 0457(T) (=DSM 44993(T)=NRRL B-24474(T)).
MeSH terms
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Actinobacteria / chemistry
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Actinobacteria / classification*
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Actinobacteria / genetics
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Actinobacteria / isolation & purification*
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Bacterial Typing Techniques
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Cluster Analysis
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DNA Fingerprinting
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DNA, Bacterial / chemistry
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DNA, Bacterial / genetics
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DNA, Ribosomal / chemistry
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DNA, Ribosomal / genetics
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Fatty Acids / analysis
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Genes, rRNA
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Genotype
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Germany
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Molecular Sequence Data
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Phylogeny
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Polymerase Chain Reaction / methods
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Quinones / analysis
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RNA, Bacterial / genetics
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RNA, Ribosomal, 16S / genetics
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Sequence Analysis, DNA
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Sequence Homology, Nucleic Acid
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Soil Microbiology*
Substances
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DNA, Bacterial
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DNA, Ribosomal
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Fatty Acids
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Quinones
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RNA, Bacterial
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RNA, Ribosomal, 16S
Associated data
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GENBANK/DQ792500
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GENBANK/DQ792501
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GENBANK/DQ792502