Soluble CD163 from activated macrophages predicts mortality in acute liver failure

J Hepatol. 2007 Nov;47(5):671-6. doi: 10.1016/j.jhep.2007.05.014. Epub 2007 Jun 21.

Abstract

Background/aims: Soluble CD163 (sCD163) is a scavenger receptor shed in serum during inflammatory activation of macrophages. We investigated if sCD163 was increased and predicted outcome in acute liver failure (ALF).

Methods: Samples from 100 consecutive patients enrolled in the U.S. ALF Study Group for whom sera were available were collected on days 1 and 3, and clinical data were obtained prospectively. sCD163 levels were determined by ELISA.

Results: The median level of sCD163 was significantly increased in ALF (21.1mg/l (range 3.6-74.9)) as compared to healthy controls (2.3mg/l (0.65-5.6), p<0.0001) and patients with stable liver cirrhosis (9.8mg/l (3.6-16.9), p=0.0002). sCD163 on day 1 correlated significantly with ALT, AST, bilirubin, and creatinine. sCD163 concentrations on day 3 were elevated in patients with fatal outcome of disease compared to spontaneous survivors, 29.0mg/l (7.2-54.0) vs. 14.6mg/l (3.5-67.2), respectively (p=0.0025). Patients that were transplanted had intermediate levels. Sensitivity and specificity at a cut-off level of 26mg/l was 62% and 81%, respectively.

Conclusions: Activated macrophages are involved in ALF resulting in a 10-fold increase in sCD163. A high level (>26mg/l) of sCD163 was significantly correlated with fatal outcome and might be used with other parameters to determine prognosis.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adult
  • Antigens, CD / analysis
  • Antigens, CD / blood*
  • Antigens, Differentiation, Myelomonocytic / analysis
  • Antigens, Differentiation, Myelomonocytic / blood*
  • CD163 Antigen
  • Chemotaxis, Leukocyte / immunology*
  • Disease Progression
  • Female
  • Humans
  • Liver / cytology
  • Liver / immunology*
  • Liver / physiopathology
  • Liver Cirrhosis / diagnosis
  • Liver Cirrhosis / immunology
  • Liver Cirrhosis / mortality
  • Liver Failure, Acute / diagnosis
  • Liver Failure, Acute / immunology*
  • Macrophages / immunology*
  • Macrophages / metabolism
  • Male
  • Monitoring, Immunologic
  • Predictive Value of Tests
  • Prognosis
  • Prospective Studies
  • ROC Curve
  • Receptors, Cell Surface / analysis
  • Receptors, Cell Surface / blood*
  • Sensitivity and Specificity
  • Solubility
  • Survival Rate
  • Up-Regulation / immunology

Substances

  • Antigens, CD
  • Antigens, Differentiation, Myelomonocytic
  • CD163 Antigen
  • Receptors, Cell Surface