Abstract
A high-speed counter-current chromatography method was developed for the separation and purification of bioactive flavonol glycosides from a crude ethanol extract of Ginkgo biloba leaves. The separation was performed with a two-phase solvent system composed of n-hexane-butanol-ethyl acetate-methanol-0.5% acetic acid (1:0.5:3.5:1:4, v/v) and three pure compounds were eluted in high purities in a one-step separation. Their purities were determined by HPLC and identified by MS,(1)H-NMR, and(13)C-NMR.
Publication types
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Evaluation Study
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Research Support, Non-U.S. Gov't
MeSH terms
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Countercurrent Distribution / instrumentation
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Countercurrent Distribution / methods*
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Flavonols* / chemistry
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Flavonols* / isolation & purification
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Ginkgo biloba / anatomy & histology
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Ginkgo biloba / chemistry*
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Glycosides* / chemistry
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Glycosides* / isolation & purification
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Medicine, Chinese Traditional
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Molecular Structure
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Nuclear Magnetic Resonance, Biomolecular
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Plant Leaves / chemistry*
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Plants, Medicinal / chemistry
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Rain
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Reproducibility of Results
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Solvents
Substances
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Flavonols
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Glycosides
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Solvents