Abstract
1. In the present study, we investigated whether the administration of apocynin, an NADPH oxidase inhibitor, provided brain protection in a rat model of intracerebral haemorrhage (ICH). 2. Rats were divided into sham, ICH untreated, ICH treated with vehicle (ethanol) and ICH treated with apocynin groups. Intracerebral haemorrhage was induced by collagenase injection. Neurological function, haemorrhage volume and brain oedema were measured 24 h after ICH. 3. Intracerebral haemorrhage caused significant neurological deficit associated with brain oedema. Apocynin (3, 10 and 30 mg/kg) failed to reduce brain injury after ICH. Low dose ethanol (0.2 g/kg) improved neurological function and reduced brain oedema (ICH-vehicle vs ICH-untreated, P < 0.05). 4. In conclusion, apocynin has no neuroprotective effect when administered intraperitoneally after ICH.
Publication types
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Research Support, N.I.H., Extramural
MeSH terms
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Acetophenones / pharmacology*
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Acetophenones / therapeutic use
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Animals
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Behavior, Animal / drug effects
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Brain / drug effects*
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Brain / enzymology
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Brain / metabolism
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Brain Edema / etiology
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Brain Edema / pathology
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Brain Edema / prevention & control
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Brain Injuries / etiology
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Brain Injuries / metabolism
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Brain Injuries / pathology
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Brain Injuries / prevention & control*
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Cerebral Hemorrhage / chemically induced
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Cerebral Hemorrhage / complications*
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Cerebral Hemorrhage / drug therapy
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Cerebral Hemorrhage / metabolism
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Cerebral Hemorrhage / pathology
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Collagenases
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Disease Models, Animal
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Dose-Response Relationship, Drug
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Enzyme Activation
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Enzyme Inhibitors / pharmacology*
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Enzyme Inhibitors / therapeutic use
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Ethanol / pharmacology*
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Ethanol / therapeutic use
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Lipid Peroxidation / drug effects
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Male
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NADPH Oxidases / antagonists & inhibitors
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NADPH Oxidases / metabolism
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Neuroprotective Agents / pharmacology*
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Neuroprotective Agents / therapeutic use
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Rats
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Rats, Sprague-Dawley
Substances
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Acetophenones
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Enzyme Inhibitors
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Neuroprotective Agents
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Ethanol
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acetovanillone
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NADPH Oxidases
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Collagenases