Abstract
It has been reported that deer antler extract has anti-bone resorptive activity in vivo. However, little is known about the cellular and molecular mechanism of this effect. In this study, we investigated the effects of deer antler extracts on osteoclast differentiation and bone-resorption in vitro. Chloroform extract (CE-C) of deer antler inhibited osteoclast differentiation in mouse bone marrow cultures stimulated by receptor activator of NF-kappaB ligand (RANKL) and macrophage-colony stimulating factor (M-CSF). CE-C suppressed the activation of extracellular signal-regulated kinase (ERK), protein kinase B (PKB/Akt) and inhibitor of kappa B (I-kappaB) by RANKL in osteoclast precursor cells. It also inhibited the bone resorptive activity of differentiated osteoclasts that was accompanied by disruption of actin rings and induction of the apoptosis. These results demonstrate deer antler extract may be a useful remedy for the treatment of bone-resorption diseases such as osteoporosis.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Actins / metabolism
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Adaptor Proteins, Signal Transducing / metabolism
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Animals
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Antlers / chemistry*
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Apoptosis / drug effects
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Bone Marrow Cells / cytology
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Bone Marrow Cells / drug effects
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Bone Marrow Cells / metabolism
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Bone Resorption / metabolism
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Bone Resorption / pathology
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Bone Resorption / prevention & control*
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Cell Differentiation / drug effects*
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Cell Survival / drug effects
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Cells, Cultured
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Chloroform / chemistry
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Deer*
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Dose-Response Relationship, Drug
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Extracellular Signal-Regulated MAP Kinases / metabolism
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Macrophages / cytology
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Macrophages / drug effects
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Macrophages / metabolism
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Mice
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Mice, Inbred C57BL
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NF-kappa B / metabolism
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Osteoblasts / cytology
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Osteoblasts / drug effects
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Osteoblasts / metabolism
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Osteoclasts / cytology
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Osteoclasts / drug effects*
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Osteoclasts / metabolism
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Phosphorylation / drug effects
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Proto-Oncogene Proteins c-akt / metabolism
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RANK Ligand / pharmacology
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Tissue Extracts / chemistry
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Tissue Extracts / isolation & purification
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Tissue Extracts / pharmacology*
Substances
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Actins
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Adaptor Proteins, Signal Transducing
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NF-kappa B
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RANK Ligand
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Tissue Extracts
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Chloroform
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Proto-Oncogene Proteins c-akt
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Extracellular Signal-Regulated MAP Kinases