Lantang pig (16 sows), which is one of the south China type pig breeds, was crossed with Landrace pig (8 boars) to construct the resource population. According to the pig linkage map of USDA-MARC2.0, 31 microsatellite DNA markers on pig chromosomes 1, 4, 7, and 8 were used to genotype the parents F1 and F2. The distance between adjacent markers was about 10 to 20 cM. The gene frequency, heterozygosity (h), and polymorphism information content (PIC) were calculated. The marker genotype of parents, F1, and F2 were obtained with WAVEO nucleotide fragment analysis system (DHPLC) and ABI 377 DNA sequencer. Twenty-one microsatellite markers on chromosomes 1, 4, and 8 were genotyped with ABI 377. The length of the DNA fragments of 18 alleles on 13 microsatellite markers were beyond the range reported on the web site. The loci of new alleles were 62% of total markers. The heterozygosities of the 31 microsatellite ranged from 0.043 to 0.7855, the heterozygosities of 70% loci were over 0.6, the average heterozygosity was 0.6460. The average polymorphism information content (PIC) of 31 microsatellite markers was 0.5949, the PIC of 77.4% loci were over 0.5. The values of h and PIC suggested that polymorphism information of these markers in the resource population was plentiful. These markers could be used to map quantitative traits loci of important economic traits in this population.