A method for the direct determination of manganese in edible fungi using suspension sample introduction and hyposensitive wavelength by flame atomic absorption spectrometry(FAAS) was developed without chemical treatment. Agar sol was used as a suspending agent. Ground and sieved edible fungi sample was suspended homogeneously in the agar sol and introduced into air-acetylene flame, and manganese in the sample was determined directly. The results showed that the method was simple, and rapid with no interference. The linear range was 0-12 microg x mL(-1). The relative standard deviation (RSD) was 0. 67% and the recovery was 97%-99%. It has been applied to the determination of manganese in edible fungi, standard boletus edulis and melanoleuca arcunta samples, and satisfactory results were achieved.