Determination of the rate constants for the FK506 binding protein/rapamycin interaction using surface plasmon resonance: an alternative sensor surface for Ni2+-nitrilotriacetic acid immobilization of His-tagged proteins

Anal Biochem. 2007 Dec 15;371(2):250-2. doi: 10.1016/j.ab.2007.06.034. Epub 2007 Jun 27.
No abstract available

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Histidine / chemistry*
  • Histidine / metabolism
  • Humans
  • Kinetics
  • Nickel / chemistry*
  • Nickel / metabolism
  • Nitrilotriacetic Acid / chemistry*
  • Nitrilotriacetic Acid / metabolism
  • Recombinant Proteins / chemistry
  • Recombinant Proteins / metabolism
  • Sirolimus / metabolism*
  • Surface Plasmon Resonance*
  • Tacrolimus Binding Proteins / chemistry*
  • Tacrolimus Binding Proteins / metabolism*

Substances

  • Recombinant Proteins
  • Histidine
  • Nickel
  • Tacrolimus Binding Proteins
  • Nitrilotriacetic Acid
  • Sirolimus