Abstract
We evaluated the effectiveness of in vivo electroporation (EP) for the enhancement of immune responses induced by DNA plasmids encoding the pre-erythrocytic Plasmodium yoelii antigens PyCSP and PyHEP17 administered intramuscularly and intradermally to mice. EP resulted in a 16- and 2-fold enhancement of antibody responses to PyCSP and PyHEP17, respectively. Immunization with 5 microg of DNA via EP was equivalent to 50 microg of DNA via conventional needle, thus reducing by 10-fold the required dose to produce a given effect. Moreover, IFN-gamma responses were increased by approximately 2-fold. Data demonstrate the potential of EP to enhance immune responses to DNA vaccines against infectious agents.
Publication types
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Research Support, U.S. Gov't, Non-P.H.S.
MeSH terms
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Animals
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Antibodies, Protozoan / immunology*
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Antigens, Protozoan / genetics
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Antigens, Protozoan / immunology
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Electroporation / methods*
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Female
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Immunity, Cellular / immunology*
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Immunization / methods
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Injections, Intradermal
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Injections, Intramuscular
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Interferon-gamma / immunology*
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Liver / immunology
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Liver / parasitology
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Malaria Vaccines / administration & dosage
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Malaria Vaccines / immunology*
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Mice
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Mice, Inbred BALB C
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Plasmids / genetics
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Plasmodium yoelii / genetics
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Plasmodium yoelii / immunology
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Vaccines, DNA / administration & dosage
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Vaccines, DNA / genetics
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Vaccines, DNA / immunology*
Substances
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Antibodies, Protozoan
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Antigens, Protozoan
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Malaria Vaccines
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Vaccines, DNA
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Interferon-gamma