Rapid diagnosis of pneumococcal pneumonia among HIV-infected adults with urine antigen detection

J Infect. 2007 Oct;55(4):300-9. doi: 10.1016/j.jinf.2007.06.014. Epub 2007 Aug 10.

Abstract

Objectives: Streptococcus pneumoniae is the leading cause of bacterial pneumonia and associated bacteremia during HIV infection. Rapid diagnostic assays may limit inappropriate therapy.

Methods: Clinical signs and symptoms and sera and urine were collected prospectively from 70 adults with pneumococcal pneumonia, including 47 with HIV co-infection. Pneumococcal C-polysaccharide antigen was detected in urine using the Binax immunochromatographic test (ICT). A systematic review of 24 published studies was conducted.

Results: Clinical symptoms, signs, and laboratory parameters except leukocytosis, were similar in HIV-infected and HIV-seronegative pneumonia. The performance of the urine antigen ICT was independent of HIV-status (sensitivity 81%, specificity 98%, positive (PPV) and negative predictive values (NPV) 98%, and 82%, respectively). The sensitivity of sputum Gram's stain was 58% (34/59) with sputum unable to be provided by 16%. The CRP response was identical in HIV-infected (mean+/-SD) 133+/-88 vs. seronegative 135+/-104 mg/L (p=0.9). In the systematic review, the ICT performance revealed 74% sensitivity (95% CI 72-77%) and 94% specificity (95% CI 93-95%). Urine antigen testing increases etiologic diagnosis by 23% (range: 10-59%) when testing adults with community acquired pneumonia of unknown etiology.

Conclusions: Urinary antigen detection provides a credible rapid diagnostic test for pneumococcal pneumonia regardless of HIV-status. CRP response to acute infection is similar in HIV co-infection and increases diagnostic certainty.

Publication types

  • Clinical Trial
  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.
  • Review
  • Systematic Review

MeSH terms

  • Adult
  • Antigens, Bacterial / urine
  • C-Reactive Protein / analysis
  • Female
  • HIV Infections / complications*
  • Humans
  • Male
  • Middle Aged
  • Pneumonia, Pneumococcal / diagnosis*
  • Pneumonia, Pneumococcal / etiology
  • Pneumonia, Pneumococcal / urine
  • Polysaccharides, Bacterial / urine*
  • Predictive Value of Tests
  • Prospective Studies
  • Urinalysis

Substances

  • Antigens, Bacterial
  • Polysaccharides, Bacterial
  • polysaccharide C-substance (Streptococcus)
  • C-Reactive Protein