Abstract
Human Dectin-1 (hDectin-1) is a member of the C-type lectin-like receptor family that was shown to be the major receptor for fungal beta-glucans and to play an important role in the cellular responses mediated by these carbohydrates. In this study, we demonstrate that hDectin-1 is involved in the uptake and cross-presentation of cellular antigens. Furthermore, activation of monocyte-derived dendritic cells (MDCs) with toll-like receptor 3 (TLR3) ligand but not with TLR2 ligand or TLR7 ligand resulted in down-regulation of hDectin-1 expression and reduced phagocytosis of apoptotic tumor cells as well as presentation of pp65-derived T-cell epitopes upon engulfment of cytomegalovirus (CMV)-infected human foreskin fibroblasts.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Antigen Presentation / immunology*
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Apoptosis
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Cell Line
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Chromium
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Cross-Priming / immunology*
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Cytomegalovirus Infections / immunology
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Dendritic Cells / cytology
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Dendritic Cells / immunology
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Fibroblasts / cytology
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Fibroblasts / virology
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Gene Expression Regulation
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HLA-A2 Antigen / immunology
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Humans
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Lectins, C-Type
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Membrane Proteins / chemistry
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Membrane Proteins / genetics
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Membrane Proteins / immunology*
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Membrane Proteins / metabolism
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Nerve Tissue Proteins / chemistry
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Nerve Tissue Proteins / genetics
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Nerve Tissue Proteins / immunology*
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Nerve Tissue Proteins / metabolism
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Phagocytosis
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Phenotype
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Protein Structure, Tertiary
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RNA, Messenger / genetics
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RNA, Messenger / metabolism
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Recombinant Proteins
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T-Lymphocytes, Cytotoxic / cytology
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T-Lymphocytes, Cytotoxic / immunology
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Toll-Like Receptors / agonists
Substances
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HLA-A2 Antigen
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Lectins, C-Type
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Membrane Proteins
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Nerve Tissue Proteins
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RNA, Messenger
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Recombinant Proteins
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Toll-Like Receptors
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dectin 1
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Chromium