Biochemical and genetics studies demonstrated the critical roles of phospholipid signaling and relevant molecules in regulating multiple processes of plant growth and development, signal transduction, mediating hormone effects and cell responses to environmental stimuli, through modulating protein subcellular localization, cross-talking with other signaling or metabolic pathways, or interacting with signaling molecules. The updated achievements of physiological effects and functional mechanism of phospholipid signaling in higher plants were reviewed.