Fine-scale genetic diversity among Burkholderia pseudomallei soil isolates in northeast Thailand

Appl Environ Microbiol. 2007 Oct;73(20):6678-81. doi: 10.1128/AEM.00986-07. Epub 2007 Aug 24.

Abstract

Burkholderia pseudomallei soil isolates from northeast Thailand were genotyped using multiple-locus variable-number tandem repeat (VNTR) analysis (MLVA) and multilocus sequence typing (MLST). MLVA identified 19 genotypes within three clades, while MLST revealed two genotypes. These close genetic relationships imply a recent colonization followed by localized expansion, similar to what occurs in an outbreak situation.

Publication types

  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Bacterial Proteins / genetics
  • Bacterial Proteins / metabolism
  • Bacterial Typing Techniques
  • Burkholderia pseudomallei / classification*
  • Burkholderia pseudomallei / genetics
  • Burkholderia pseudomallei / isolation & purification
  • DNA, Bacterial / analysis
  • Genetic Variation*
  • Genotype
  • Minisatellite Repeats / genetics
  • Soil Microbiology*
  • Thailand

Substances

  • Bacterial Proteins
  • DNA, Bacterial