Primary structure and localization of a conserved immunogenic Plasmodium falciparum glutamate rich protein (GLURP) expressed in both the preerythrocytic and erythrocytic stages of the vertebrate life cycle

Mol Biochem Parasitol. 1991 Nov;49(1):119-31. doi: 10.1016/0166-6851(91)90135-s.

Abstract

A gene coding for a 220-kDa glutamate rich protein (GLURP), an exoantigen of Plasmodium falciparum, was isolated and its nucleotide sequence was determined. The deduced amino acid sequence contains 2 repeat regions. The sequence of one of these was shown to be conserved among geographically dispersed isolates, and a fusion protein containing that sequence was able to stimulate B- and T-cells. Antibodies against GLURP stained erythrocytic stages of the parasite as well as the hepatic stage as detected by electron microscopy.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Antigens, Protozoan / genetics*
  • Base Sequence
  • Chromosome Mapping
  • DNA, Protozoan / genetics
  • Erythrocytes / parasitology
  • Microscopy, Immunoelectron
  • Molecular Sequence Data
  • Plasmodium falciparum / genetics*
  • Plasmodium falciparum / growth & development
  • Plasmodium falciparum / immunology
  • Protozoan Proteins / genetics*
  • Protozoan Proteins / immunology

Substances

  • Antigens, Protozoan
  • DNA, Protozoan
  • Protozoan Proteins
  • glutamate-rich protein, Plasmodium

Associated data

  • GENBANK/M59706
  • GENBANK/M61207
  • GENBANK/M61208
  • GENBANK/S74964
  • GENBANK/S74965
  • GENBANK/S74966
  • GENBANK/S75014
  • GENBANK/S75016
  • GENBANK/S77801
  • GENBANK/S78950
  • GENBANK/X57167