Characterization of a new HLA-B allele, HLA-B*5312, and re-evaluation of the published sequences of the untranslated regions of HLA-B*35 and HLA-B*53

A Heinold; M Bauer; S Scherer; G Opelz; T H Tran

doi:10.1111/j.1399-0039.2007.00900.x

Characterization of a new HLA-B allele, HLA-B5312, and re-evaluation of the published sequences of the untranslated regions of HLA-B35 and HLA-B*53

Tissue Antigens. 2007 Oct;70(4):319-23. doi: 10.1111/j.1399-0039.2007.00900.x.

Authors

A Heinold¹, M Bauer, S Scherer, G Opelz, T H Tran

Affiliation

¹ Department of Transplantation Immunology, Institute of Immunology, University of Heidelberg, Im Neuenheimer Feld 305, 69120 Heidelberg, Germany. [email protected]

PMID: 17767554
DOI: 10.1111/j.1399-0039.2007.00900.x

Abstract

We report a novel human leukocyte antigen (HLA)-B allele, HLA-B*5312. Compared with HLA-B*530101, there is one silent substitution at nucleotide 438 and two non-synonymous substitutions at nucleotides 431 and 440, causing a change of the amino acid sequence (Asn-->Ser at codon 77 and Ile-->Thr at codon 80, respectively) within the Bw4 epitope. In contrast to the published sequences (IMGT/HLA Database, version 2.16.0, January 2007), we found that HLA-B*530101 had a C instead of a T at nucleotide -221, whereas HLA-B*350101 had a C instead of an A at nucleotide 2992. According to our sequencing results, HLA-B*5312 resembles HLA-B*350101 regarding its sequence of the untranslated regions. HLA-B*5312 may have been the result of a double crossing over event during which HLA-B*350101 adopted a Bw4 motif.

MeSH terms

Alleles*
Base Sequence
HLA-B Antigens / genetics*
Humans
Molecular Sequence Data
Untranslated Regions

Substances

HLA-B Antigens
Untranslated Regions

Associated data

GENBANK/AM292927