Abstract
Immunoproteomic approaches were undertaken to study the immunogenicity of the membrane-associated proteins of the Streptococcus suis type 2 (SS2) China vaccine strain HA9801. The membrane-associated proteins were enriched using the Triton X-114 extraction protocol and were analysed by two-dimensional gel electrophoresis (2-DE) and subsequent immunoblotting using the hyperimmune serum of SS2-HA9801-immunized specific pathogen free (SPF) minipigs. A total of 11 proteins were recognized, and the corresponding spots on a duplicate gel were excised and identified by MALDI-TOF MS.
MeSH terms
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Animals
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Bacterial Proteins / immunology*
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Bacterial Vaccines / immunology*
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Blotting, Western / veterinary
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China
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Electrophoresis, Gel, Two-Dimensional / veterinary
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Electrophoresis, Polyacrylamide Gel / veterinary
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Random Allocation
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Specific Pathogen-Free Organisms
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Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / veterinary
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Streptococcal Infections / prevention & control
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Streptococcal Infections / veterinary*
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Streptococcus suis / immunology*
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Swine
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Swine Diseases / prevention & control*
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Swine, Miniature*
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Vaccination / veterinary
Substances
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Bacterial Proteins
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Bacterial Vaccines