Nocardia tenerifensis genome identification in a cutaneous granuloma of a cat

J Vet Diagn Invest. 2007 Sep;19(5):577-80. doi: 10.1177/104063870701900522.

Abstract

An adult Domestic Longhair cat developed a subcutaneous mass in its tail. Histologically, this mass consisted of ill-defined pyogranulomas centered around aggregates of gram-positive, acid-fast filamentous bacteria, consistent with Nocardia. Due to the lack of fresh samples, DNA was extracted from formalin-fixed paraffin-embedded tissue sections and subjected to polymerase chain reaction amplification and DNA sequencing of 16S ribosomal RNA gene encompassing Nocardia sp.-specific sequences. Sequences analyzed using the GenBank database revealed 99.5% homology with Nocardia spp. and had the highest sequence homology of 98.2% with Nocardia tenerifensis among Nocardia spp. To the authors' knowledge, this is the first report of detection of N. tenerifensis genome associated with cutaneous nocardiosis in an animal.

Publication types

  • Case Reports

MeSH terms

  • Animals
  • Cat Diseases / microbiology*
  • Cat Diseases / pathology
  • Cats
  • Genome, Bacterial*
  • Granuloma / microbiology
  • Granuloma / pathology
  • Granuloma / veterinary*
  • Male
  • Nocardia / classification
  • Nocardia / genetics*
  • Nocardia / isolation & purification*
  • Nocardia Infections / microbiology
  • Nocardia Infections / pathology
  • Nocardia Infections / veterinary*
  • Skin Diseases, Bacterial / microbiology
  • Skin Diseases, Bacterial / pathology
  • Skin Diseases, Bacterial / veterinary*
  • Tail / pathology