Abstract
The purB and purH mutants of Mesorhizobium loti exhibited purine auxotrophy and nodulation deficiency on Lotus japonicus. In the presence of adenine, only the purH mutant induced nodule formation and the purB mutant produced few infection threads, suggesting that 5-aminoimidazole-4-carboxamide ribonucleotide biosynthesis catalyzed by PurB is required for the establishment of symbiosis.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Bacterial Proteins / genetics*
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Bacterial Proteins / metabolism
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Gene Expression Regulation, Bacterial
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Genes, Bacterial
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Lotus / metabolism*
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Lotus / microbiology*
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Mutation
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Rhizobiaceae / genetics*
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Rhizobiaceae / metabolism
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Ribonucleotides / biosynthesis
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Root Nodules, Plant / growth & development*
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Root Nodules, Plant / microbiology*
Substances
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Bacterial Proteins
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Ribonucleotides