It has been recognised that ultrasonic files leave a diagonal pattern within the canal following endosonic instrumentation. There is confusion on the causative factor on the production of these marks. Furthermore there is little information about the marks produced by sonic files. An in vitro system consisting of highly polished dentin discs demonstrated that the ultrasonic K-file made a diagonal pattern that was an imprint of the cutting edges of the file. The sonic files produced different markings which were the result of their longitudinal and transverse motion. Root canals of extracted teeth were prepared with these instruments and similar grooves were observed. The ultrasonic K-file had diagonal grooves in the coronal and middle third of the root canal but longitudinal marks in the apical third. The rispisonic and shaper files produced debris even in the presence of NaOCl. This debris was formed into discrete parallel bands. Both longitudinal and transverse grooves were present and the canal had many other scratches similar to those seen with the dentin discs. There were differences between the marks produced by either ultrasonic or sonic endosonic instruments but it is unknown whether this influences the long term prognosis of the root canal therapy.