The jump performance of ten youth soccer players (mean age 15.8 years, s= 0.4) was assessed before and after 42 min of soccer-specific exercise performed on a non-motorized treadmill. A squat, countermovement, and drop jump were performed on a force platform and simultaneously surface EMG activity of four lower limb muscles was collected. Jump height deteriorated across all conditions with mean reductions of - 1.4 cm (s = 1.6; P < 0.05), - 3.0 cm (s = 2.9; P < 0.05), and -2.3 cm (s = 1.7; P < 0.01) in the squat, countermovement, and drop jump respectively. The impact force in the drop jump was the only force variable to show a significant change with fatigue (P < 0.05). Following the prolonged exercise, reductions in total muscle activity were non-significant for the squat jump, approached significance for the counter-ovement jump (P = 0.07), and achieved significance for the drop jump (P < 0.05). The results showed that completing soccer-specific exercise reduced performance in all jump tasks. Reductions in muscle activity were greatest for the drop jump, suggesting an influence of muscle stretch and loading on reduced muscle activity when fatigued.