Abstract
Anti-hypertensive agents with antioxidative effects are potentially useful for diabetic patients with hypertension to prevent the onset and progression of their complication. While dihydropyridine-type calcium antagonists are among the frequently used anti-hypertensive drugs, azelnidipine, a novel calcium antagonist, has been reported to have a unique anti-oxidative effect in vitro and in animals. In this study, we measured lipid hydroperoxides in human sample using diphenyl-1-pyrenylphosphine for the first time, and used the value of lipid hydroperoxides as an index of oxidative stress. Then, we compared the antioxidative properties of azelnidipine and amlodipine, a frequently used calcium antagonist in hypertensive diabetic patients. Administration of vitamin C and E for 8 weeks significantly reduced lipid hydroperoxides in erythrocyte membrane in normal subjects. In hypertensive diabetic patients, azelnidipine treatment for 12 weeks induced a more significant fall in erythrocyte lipid hydroperoxide level than amlodipine, though blood pressure during each treatment was comparable. Our data confirm the usefulness of lipid hydroperoxides in erythrocyte membrane as a marker of oxidative stress in vivo, and indicate that azelnidipine has a unique antioxidative property in human.
Publication types
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Comparative Study
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Controlled Clinical Trial
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Research Support, Non-U.S. Gov't
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Validation Study
MeSH terms
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Adult
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Aged
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Amlodipine / therapeutic use
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Antihypertensive Agents / pharmacology
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Antihypertensive Agents / therapeutic use
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Ascorbic Acid / administration & dosage
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Azetidinecarboxylic Acid / analogs & derivatives*
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Azetidinecarboxylic Acid / pharmacology
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Azetidinecarboxylic Acid / therapeutic use
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Blood Pressure / drug effects*
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Calcium Channel Blockers / pharmacology
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Calcium Channel Blockers / therapeutic use
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Diabetes Mellitus, Type 2 / complications
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Diabetes Mellitus, Type 2 / metabolism*
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Diabetes Mellitus, Type 2 / physiopathology*
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Dihydropyridines / pharmacology*
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Dihydropyridines / therapeutic use*
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Erythrocytes / chemistry
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Erythrocytes / drug effects
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Female
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Humans
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Hypertension / complications
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Hypertension / drug therapy*
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Lipid Peroxides / analysis
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Lipid Peroxides / metabolism*
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Male
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Middle Aged
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Organophosphorus Compounds / chemistry
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Organophosphorus Compounds / pharmacology
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Pyrenes / chemistry
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Pyrenes / pharmacology
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Vitamin E / administration & dosage
Substances
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Antihypertensive Agents
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Calcium Channel Blockers
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Dihydropyridines
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Lipid Peroxides
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Organophosphorus Compounds
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Pyrenes
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diphenyl-1-pyrenylphosphine
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Vitamin E
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Amlodipine
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Azetidinecarboxylic Acid
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Ascorbic Acid
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azelnidipine