Preliminary X-ray crystallographic studies of a Lys49-phospholipase A2 homologue from Bothrops pirajai venom complexed with p-bromo-phenacyl bromide and alpha-tocopherol inhibitors

Juliana I Dos Santos; Daniela P Marchi-Salvador; Carlos A H Fernandes; Lucas B Silveira; Andreimar M Soares; Marcos R M Fontes

doi:10.2174/092986607781483796

Preliminary X-ray crystallographic studies of a Lys49-phospholipase A2 homologue from Bothrops pirajai venom complexed with p-bromo-phenacyl bromide and alpha-tocopherol inhibitors

Protein Pept Lett. 2007;14(7):698-701. doi: 10.2174/092986607781483796.

Authors

Juliana I Dos Santos¹, Daniela P Marchi-Salvador, Carlos A H Fernandes, Lucas B Silveira, Andreimar M Soares, Marcos R M Fontes

Affiliation

¹ Departamento de Física e Biofísica, Instituto de Biociências, UNESP, C. P. 510, CEP 18618-000, Botucatu-SP, Brazil.

PMID: 17897096
DOI: 10.2174/092986607781483796

Abstract

PrTX-I, a non-catalytic and myotoxic Lys49-PLA(2) from Bothrops pirajai venom has been crystallized alone and in complex with bromophenacyl bromide (BPB), alpha tocopherol and alpha tocopherol acetate inhibitors. These crystals have shown to diffract X-rays between 2.34 and 1.65 A resolution. All complexes crystals are isomorphous and belong to the space group P2(1) whereas native PrTX-I crystals belong to the P3(1)21.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Bothrops
Bromides / pharmacology*
Crotalid Venoms / chemistry*
Crystallography, X-Ray
Enzyme Inhibitors / pharmacology*
Lysine / chemistry*
Models, Molecular
Phospholipases A / chemistry*
Protein Conformation
alpha-Tocopherol / pharmacology*

Substances

Bromides
Crotalid Venoms
Enzyme Inhibitors
Phospholipases A
alpha-Tocopherol
Lysine