Abstract
A novel real-time PCR assay for detection of human papillomavirus type 52 (HPV-52) DNA (RT-52) was evaluated on 265 anogenital samples. RT-52 had a sensitivity of 98.4% and a specificity of 100% compared to conventional HPV-52 typing assays, including hybridization of PGMY products with an HPV-52-specific probe and PCR sequencing of HPV-52 E6.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Anal Canal / virology*
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DNA, Viral / analysis
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Female
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Genitalia / virology*
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Genotype
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Humans
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Male
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Oligonucleotide Array Sequence Analysis
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Papillomaviridae / genetics
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Papillomaviridae / isolation & purification*
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Papillomavirus Infections / diagnosis*
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Polymerase Chain Reaction / methods*
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Sensitivity and Specificity