Circulating neutrophil CD14 expression and the inverse association of ambient particulate matter on lung function in asthmatic children

Ann Allergy Asthma Immunol. 2007 Sep;99(3):244-53. doi: 10.1016/S1081-1206(10)60660-6.

Abstract

Background: Identifying baseline inflammatory biomarkers that predict susceptibility to size-specific particulate matter (PM) independent of gaseous pollutants could help us better identify asthmatic subpopulations at increased risk for the adverse health effects of PM.

Objective: To evaluate whether the association between lung function and exposure to ambient levels of PM less than 2.5 microm in diameter (PM2.5) (fine) and 10 to 2.5 microm in diameter (PM(10-2.5)) (coarse) in children with persistent asthma differed across baseline measures of inflammation and innate immune activation.

Methods: We performed a panel study on a local population of 16 children with persistent asthma and evaluated daily pulmonary function (percentage of predicted peak expiratory flow and forced expiratory volume in 1 second) while concurrently measuring daily PM2.5 and PM(10-2.5) exposure from a central site in Chapel Hill, North Carolina. The children underwent a baseline medical evaluation that included assessment of several immunoinflammatory biomarkers in peripheral blood.

Results: Children without measurable CD14 expression on circulating neutrophils had significantly reduced pulmonary function (forced expiratory volume in 1 second and peak expiratory flow) with each interquartile range (IQR) increase in PM2.5 (IQR = 8.5 microg/m3) and PM(10-2.5) (IQR = 4.1 microg/m3) concentration, unlike children with measurable CD14 expression (P < .001 for interaction).

Conclusions: Asthmatic children with muted surface expression of CD14 on circulating neutrophils may have a decreased capacity to respond to bacterial components of PM.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Adolescent
  • Antigens, CD / analysis
  • Antigens, CD / blood
  • Asthma / immunology*
  • Asthma / physiopathology
  • Biomarkers / blood
  • Blood Cell Count
  • Breath Tests
  • Child
  • Female
  • Forced Expiratory Volume
  • Humans
  • Lipopolysaccharide Receptors / analysis*
  • Lipopolysaccharide Receptors / blood
  • Lung / immunology*
  • Lung / physiopathology
  • Male
  • Nasal Lavage Fluid / cytology
  • Neutrophils / chemistry
  • Neutrophils / cytology
  • Neutrophils / immunology*
  • North Carolina
  • Particulate Matter / adverse effects
  • Particulate Matter / analysis
  • Particulate Matter / immunology*
  • Peak Expiratory Flow Rate
  • Respiratory Function Tests
  • Spirometry
  • Temperature

Substances

  • Antigens, CD
  • Biomarkers
  • Lipopolysaccharide Receptors
  • Particulate Matter