Altererythrobacter epoxidivorans gen. nov., sp. nov., an epoxide hydrolase-active, mesophilic marine bacterium isolated from cold-seep sediment, and reclassification of Erythrobacter luteolus Yoon et al. 2005 as Altererythrobacter luteolus comb. nov

Int J Syst Evol Microbiol. 2007 Oct;57(Pt 10):2207-2211. doi: 10.1099/ijs.0.64863-0.

Abstract

A novel marine bacterium, strain JCS350(T), was isolated from marine sediment samples collected from a cold-seep area. The 16S rRNA gene sequence of the isolate showed high similarity to that of Erythrobacter luteolus SW-109(T) (95.9 % sequence similarity). Lower 16S rRNA gene sequence similarities were shown to other members of the genus Erythrobacter (94.6-95.4 %) and members of the genus Porphyrobacter (94.5-95.2 %). Phylogenetic analysis with all members of the family Erythrobacteraceae and several members of the family Sphingomonadaceae revealed that the isolate formed a phyletic line with [Erythrobacter] luteolus that was distinct from other members of the family Erythrobacteraceae. The dominant fatty acids of strain JCS350(T) were 18 : 1omega7c, 16 : 1omega7c and cyclopropane 17 : 0. The major respiratory quinone was ubiquinone 10. The DNA G+C content was 54.5 mol%. The isolate did not contain bacteriochlorophyll a. Optimal growth required the presence of 2 % (w/v) NaCl with either 0.18 % CaCl(2) or 0.59 % MgCl(2), at pH 6.5 and at 35 degrees C. On the basis of the evidence of this polyphasic taxonomic study, strain JCS350(T) should be classified in a novel genus and species in the family Erythrobacteraceae, for which the name Altererythrobacter epoxidivorans gen. nov., sp. nov. is proposed. The misclassified species [Erythrobacter] luteolus is transferred to the new genus as Altererythrobacter luteolus comb. nov. The type strain of Altererythrobacter epoxidivorans is JCS350(T) (=KCCM 42314(T) =JCM 13815(T)) and the type strain of Altererythrobacter luteolus is SW-109(T) (=KCTC 12311(T) =JCM 12599(T)).

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Alphaproteobacteria / classification*
  • Alphaproteobacteria / enzymology
  • Alphaproteobacteria / genetics
  • Alphaproteobacteria / isolation & purification*
  • Bacteriochlorophyll A / analysis
  • Base Composition
  • Calcium Chloride / metabolism
  • DNA, Bacterial / chemistry
  • DNA, Bacterial / genetics
  • DNA, Ribosomal / chemistry
  • DNA, Ribosomal / genetics
  • Epoxide Hydrolases / metabolism*
  • Fatty Acids / analysis
  • Genes, rRNA
  • Geologic Sediments / microbiology*
  • Hydrogen-Ion Concentration
  • Magnesium Chloride / metabolism
  • Molecular Sequence Data
  • Phylogeny
  • Quinones / analysis
  • RNA, Bacterial / genetics
  • RNA, Ribosomal, 16S / genetics
  • Sequence Analysis, DNA
  • Sequence Homology, Nucleic Acid
  • Sodium Chloride / metabolism
  • Temperature

Substances

  • Bacteriochlorophyll A
  • DNA, Bacterial
  • DNA, Ribosomal
  • Fatty Acids
  • Quinones
  • RNA, Bacterial
  • RNA, Ribosomal, 16S
  • Magnesium Chloride
  • Sodium Chloride
  • Epoxide Hydrolases
  • Calcium Chloride

Associated data

  • GENBANK/DQ304436