[Cathepsin D expression in ovaries from polycystic ovarian syndrome patients]

Min Jin; Jie Cai; Yan-jun Hu; Xiu-e Lu; He-feng Huang

doi:10.3785/j.issn.1008-9292.2007.05.003

[Cathepsin D expression in ovaries from polycystic ovarian syndrome patients]

Zhejiang Da Xue Xue Bao Yi Xue Ban. 2007 Sep;36(5):429-32. doi: 10.3785/j.issn.1008-9292.2007.05.003.

[Article in Chinese]

Authors

Min Jin¹, Jie Cai, Yan-jun Hu, Xiu-e Lu, He-feng Huang

Affiliation

¹ The Affiliated Obstetrics and Gynecology Hospital, College of Medicine, Zhejiang University, Hangzhou 310006, China.

PMID: 17924458
DOI: 10.3785/j.issn.1008-9292.2007.05.003

Abstract

Objective: To investigate the expression of cathepsin D in ovary of patients with polycystic ovarian syndrome (PCOS).

Methods: Western blot was performed to detect the expression of cathepsin D and immunohistochemistry was used to detect the protein distribution in ovarian tissue.

Result: Semi-quantity values of cathepsin D expression in PCOS and control group were 2.06 +/- 0.39 and 4.76 +/- 1.43 (P<0.05), respectively. Immunostaining for cathepsin D was obvious in both follicles and stromal cells, and the strongest immunostaining was seen in granulosa cells of follicles. Immunochemical study showed the protein was mainly located on the cytoplasm and cell membrane.

Conclusion: Cathepsin D expression is down-regulated in ovaries of PCOS patients, which may provide a clue for the abnormality of follicle development in PCOS.

Publication types

English Abstract
Research Support, Non-U.S. Gov't

MeSH terms

Adult
Blotting, Western
Cathepsin D / biosynthesis*
Down-Regulation
Female
Humans
Immunohistochemistry
Ovary / enzymology*
Ovary / pathology
Polycystic Ovary Syndrome / enzymology*

Substances

Cathepsin D