Abstract
Intact pathogenic Mycoplasma hyopneumoniae at 100 microg protein ml(-1) induced transcription of proinflammatory cytokines such as cyclooxygenase (COX)-2, tumor necrosis factor (TNF)-alpha, interleukin(IL)-1, IL-6 and inducible nitric oxide synthase (iNOS) in RAW 264.7 cells. After pretreatment with 50 microg surfactin C/ml, purified from Bacillus subtilis, transcription of the COX-2, IL-1beta, IL-6 and iNOS genes induced by M. hyopneumoniae was inhibited by 43%, 82%, 72% and 59%, respectively.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Antibiotics, Antineoplastic / pharmacology
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Cell Line
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Cells, Cultured / drug effects
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Inflammation / immunology*
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Lipopeptides
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Mice
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Mycoplasma hyopneumoniae / immunology*
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Nitric Oxide / biosynthesis*
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Nitric Oxide / genetics
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Peptides, Cyclic / pharmacology*
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RNA, Messenger / biosynthesis
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Transcription, Genetic / drug effects
Substances
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Antibiotics, Antineoplastic
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Lipopeptides
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Peptides, Cyclic
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RNA, Messenger
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surfactin peptide
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Nitric Oxide