Abstract
Mounting evidence has suggested that paeonol possesses plenty of pharmacologic actions. Our research is to determine if paeonol can protect cultured rat hippocampal neurons from oxygen-glucose deprivation(OGD)-induced injury and elucidate the underlying mechanism. We cultivated the rat hippocampal neurons as the object of study and then established the model of oxygen-glucose deprivation. Neuronal viability was measured by the reduction of 3-(4,5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide (MTT), while intracellular Ca(2+) concentration was observed by fluorospectrophotometer. The binding force of N-methyl-D-aspartate (NMDA) receptor was evaluated by liquid scintillation counting. Compared with oxygen-glucose deprivation group, paeonol treatment obviously increased cell survival rate and reduced the activity of the binding force of NMDA receptors, reversing the overload of intracellular Ca(2+). These results demonstrate that paeonol protected rat neurons from oxygen-glucose deprivation-induced injury, resulting in alleviating the morphological damage and increasing neuron viability and suggest that paeonol may exhibit its protective effect against oxygen-glucose deprivation-induced injury by targeting on NMDA receptors.
MeSH terms
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Acetophenones / pharmacology*
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Acetophenones / therapeutic use
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Animals
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Animals, Newborn
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Brain Infarction / drug therapy*
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Brain Infarction / physiopathology
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Brain Infarction / prevention & control
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Cell Survival / drug effects
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Cell Survival / physiology
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Cells, Cultured
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Cytoprotection / physiology
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Excitatory Amino Acid Antagonists / pharmacology
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Excitatory Amino Acid Antagonists / therapeutic use
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Hippocampus / blood supply
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Hippocampus / drug effects
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Hippocampus / physiopathology
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Hypoxia-Ischemia, Brain / drug therapy*
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Hypoxia-Ischemia, Brain / metabolism
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Hypoxia-Ischemia, Brain / physiopathology
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Indicators and Reagents
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Ligands
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Nerve Degeneration / drug therapy*
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Nerve Degeneration / physiopathology
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Nerve Degeneration / prevention & control
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Neurons / drug effects
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Neurons / metabolism
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Neurons / pathology
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Neuroprotective Agents / pharmacology*
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Neuroprotective Agents / therapeutic use
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Neurotoxins / antagonists & inhibitors
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Neurotoxins / metabolism
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Rats
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Rats, Wistar
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Receptors, N-Methyl-D-Aspartate / drug effects
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Receptors, N-Methyl-D-Aspartate / metabolism
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Spectrometry, Fluorescence
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Tetrazolium Salts
Substances
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Acetophenones
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Excitatory Amino Acid Antagonists
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Indicators and Reagents
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Ligands
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Neuroprotective Agents
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Neurotoxins
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Receptors, N-Methyl-D-Aspartate
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Tetrazolium Salts
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paeonol