Localization of proteins and organelles using fluorescence microscopy

Methods Mol Biol. 2007:389:239-50. doi: 10.1007/978-1-59745-456-8_17.

Abstract

This chapter describes the different methods used for localization of proteins and organelles in Pichia pastoris. A series of plasmids and a modified immunofluorescence protocol for localization and co-localization of proteins and organelles are described. Also included are protocols for the labeling of different subcellular organelles with vital stains.

Publication types

  • Research Support, N.I.H., Extramural

MeSH terms

  • Cell Nucleus / metabolism
  • Fluorescent Antibody Technique
  • Indoles
  • Microbial Viability
  • Microscopy, Fluorescence
  • Mitochondria / metabolism
  • Organelles / metabolism*
  • Pichia / cytology*
  • Pichia / metabolism*
  • Protein Transport
  • Proteins / metabolism*
  • Vacuoles / metabolism

Substances

  • Indoles
  • Proteins
  • DAPI