Transformation of Drosophila cell lines: an alternative approach to exogenous protein expression

Lucy Cherbas; Peter Cherbas

doi:10.1007/978-1-59745-457-5_16

Transformation of Drosophila cell lines: an alternative approach to exogenous protein expression

Methods Mol Biol. 2007:388:317-40. doi: 10.1007/978-1-59745-457-5_16.

Authors

Lucy Cherbas¹, Peter Cherbas

Affiliation

¹ Department of Biology and Drosophila Genomics Resource Center, Indiana University, Bloomington, IN, USA.

PMID: 17951778
DOI: 10.1007/978-1-59745-457-5_16

Abstract

Techniques and experimental applications are described for exogenous protein expression in Drosophila cell lines. Ways in which the Drosophila cell lines and the baculovirus expression vector system differ in their applications are emphasized.

MeSH terms

Animals
Baculoviridae / genetics*
Cell Line
Drosophila / cytology
Drosophila / genetics
Drosophila / metabolism*
Gene Expression
Genetic Vectors / genetics
Green Fluorescent Proteins / genetics
Green Fluorescent Proteins / metabolism
Plasmids / genetics
Promoter Regions, Genetic / genetics
Recombinant Fusion Proteins / genetics
Recombinant Fusion Proteins / metabolism*
Transfection / methods

Substances

Recombinant Fusion Proteins
Green Fluorescent Proteins