Abstract
cSM5-1 is a mouse-human chimeric antibody which has a high specificity for hepatocellular carcinoma, melanoma and breast cancer. In this study, cSM5-1 was found to be able to inhibit cell growth and induce apoptosis in hepatocellular carcinoma cells. The antitumor activity of cSM5-1 was closely correlated with the expression level of the SM5-1 binding protein in the cancer cells. The role of caspases in cSM5-1-induced apoptosis was also investigated, indicating that cSM5-1-induced apoptosis was partially caspase-dependent and caspase-10 played a critical role. These in vitro data indicate that cSM5-1 has the potential to be a promising candidate for cancer treatment.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Antibodies, Monoclonal / chemistry
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Antibodies, Monoclonal / metabolism
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Antibodies, Monoclonal / pharmacology*
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Apoptosis / drug effects*
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Blotting, Western
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Carcinoma, Hepatocellular / metabolism
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Carcinoma, Hepatocellular / pathology
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Carrier Proteins / metabolism
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Caspase 10 / metabolism
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Caspase Inhibitors
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Caspases / metabolism*
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Cell Line, Tumor
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Cell Proliferation / drug effects*
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Cysteine Proteinase Inhibitors / pharmacology
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Dose-Response Relationship, Drug
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Humans
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Immunoprecipitation
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Liver Neoplasms / metabolism
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Liver Neoplasms / pathology
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Mice
Substances
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Antibodies, Monoclonal
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Carrier Proteins
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Caspase Inhibitors
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Cysteine Proteinase Inhibitors
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Caspase 10
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Caspases