Local stabilization of microtubule assembly improves recovery of facial nerve function after repair

Exp Neurol. 2008 Jan;209(1):131-44. doi: 10.1016/j.expneurol.2007.09.016. Epub 2007 Sep 26.

Abstract

Within a recent study on the recovery of vibrissae motor performance after facial nerve repair in blind (strain SD/RCS) and sighted (strain SD) rats, we found that, despite persisting myotopic disorganization in the facial nucleus, the blind animals fully restored vibrissal whisking. Searching for the morphological substrates of this improved recovery, we compared the amount of cytoskeletal proteins in the leading edge of elongating axons between both strains. Since our results showed an enhanced expression of neuronal class III beta-tubulin in the blind rats, we wondered whether this was due to an increased synthesis or to a delayed turnover of microtubules. In the present report, we approached this question applying established pharmacological agents to the transected buccal branch of the facial nerve in sighted Wistar rats perturbing either microtubule assembly towards stabilization (enhanced polymerization with 10 microg/ml taxol) or towards increased synthesis (challenged by destabilization with 100 microg/ml nocodazole and 20 microg/ml vinblastine). Evaluation of the effect(s) 2 months later included estimation of (i) vibrissae motor performance by video-based motion analysis, (ii) the degree of collateral axonal branching by double retrograde neuronal labeling with crystals of Fluoro-Gold and DiI and (iii) the pattern of motor end-plate reinnervation (proportions of mono- and poly-reinnervated) in the largest extrinsic vibrissal muscle, the m. levator labii superioris. We found that only stabilization of microtubules with 10 microg/ml taxol reduced intramuscular axonal sprouting and polyinnervation of the motor end-plates, which was accompanied by improved restoration of function.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Antineoplastic Agents / pharmacology
  • Antineoplastic Agents, Phytogenic / pharmacology
  • Axons / drug effects
  • Axons / physiology
  • Cell Count
  • Cheek / innervation
  • Data Interpretation, Statistical
  • Facial Muscles / innervation
  • Facial Muscles / physiology
  • Facial Nerve / metabolism
  • Facial Nerve / pathology*
  • Facial Nerve / surgery*
  • Female
  • Image Processing, Computer-Assisted
  • Microscopy, Fluorescence
  • Microtubules / drug effects
  • Microtubules / physiology*
  • Motor Endplate / physiology
  • Motor Endplate / ultrastructure
  • Movement / physiology
  • Neurosurgical Procedures
  • Nocodazole / pharmacology
  • Paclitaxel / pharmacology
  • Rats
  • Rats, Wistar
  • Tissue Fixation
  • Tubulin / metabolism
  • Vibrissae / physiology
  • Vinblastine / pharmacology

Substances

  • Antineoplastic Agents
  • Antineoplastic Agents, Phytogenic
  • Tubulin
  • Vinblastine
  • Paclitaxel
  • Nocodazole