Kinetic evidence that the sodium-dependent high-affinity and the sodium-independent low-affinity dopamine uptake are mediated by one carrier

Biomed Biochim Acta. 1991;50(9):1093-7.

Abstract

In synaptosomes of the rat striatum the dopamine uptake was measured in a concentration range of 0.03 microM to 100 microM. In the presence of sodium the uptake exhibited a non-Michaelis-Menten kinetics and in a sodium-free medium the uptake kinetics was sigmoid. According to these findings a novel model for the dopamine uptake is proposed. Its main assumption is one carrier with two dopamine binding sites.

MeSH terms

  • Animals
  • Binding Sites
  • Biological Transport
  • Corpus Striatum
  • Dopamine / pharmacology*
  • Kinetics
  • Male
  • Rats
  • Rats, Inbred Strains
  • Sodium / physiology*
  • Synaptosomes / drug effects
  • Synaptosomes / metabolism

Substances

  • Sodium
  • Dopamine