Eucaryotic diversity in a hypersaline microbial mat

Leah M Feazel; John R Spear; Alicia B Berger; J Kirk Harris; Daniel N Frank; Ruth E Ley; Norman R Pace

doi:10.1128/AEM.01448-07

Eucaryotic diversity in a hypersaline microbial mat

Appl Environ Microbiol. 2008 Jan;74(1):329-32. doi: 10.1128/AEM.01448-07. Epub 2007 Nov 9.

Authors

Leah M Feazel¹, John R Spear, Alicia B Berger, J Kirk Harris, Daniel N Frank, Ruth E Ley, Norman R Pace

Affiliation

¹ Department of Molecular, Cellular and Developmental Biology, University of Colorado, Boulder, CO 90309-0347, USA.

Abstract

To determine the eucaryotic diversity of the hypersaline Guerrero Negro microbial mat, we amplified 18S rRNA genes from DNA extracted from this mat and constructed and analyzed clone libraries. The extent of eucaryotic diversity detected was remarkably low, only 15 species among 890 clones analyzed. Six eucaryotic kingdoms were represented, as well as a novel cluster of sequences. Nematode sequences dominated the clone libraries.

Publication types

Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

Biodiversity*
DNA, Ribosomal / chemistry
DNA, Ribosomal / genetics
DNA, Ribosomal / isolation & purification
Eukaryotic Cells / classification*
Geologic Sediments*
Molecular Sequence Data
Sequence Analysis, DNA

Substances

DNA, Ribosomal

Associated data

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