Abstract
The formation of protein aggregates can be a major problem during the preparation of antibody-drug conjugates. Herein is described the methods by which reaction additives were selected, which reduce the tendency of antibodies to aggregate during the attachment of the cytotoxic agent calicheamicin to form an immunoconjugate. Reaction conditions were delineated that produced optimized yields of monomeric conjugates. These conditions were used in the preclinical preparations of gemtuzumab ozogamicin (Mylotarg), the first commercially available chemotherapeutic immunoconjugate.
MeSH terms
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Aminoglycosides / chemical synthesis*
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Aminoglycosides / chemistry
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Aminoglycosides / immunology*
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Antibodies, Monoclonal / chemistry
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Antibodies, Monoclonal / immunology*
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Antibodies, Monoclonal, Humanized
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Antigens, CD / immunology
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Antigens, Differentiation, Myelomonocytic / immunology
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Gemtuzumab
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Humans
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Immunoglobulin G / immunology
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Immunotoxins / chemistry*
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Immunotoxins / immunology
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Organic Chemicals / chemistry
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Sialic Acid Binding Ig-like Lectin 3
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United States
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United States Food and Drug Administration
Substances
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Aminoglycosides
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Antibodies, Monoclonal
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Antibodies, Monoclonal, Humanized
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Antigens, CD
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Antigens, Differentiation, Myelomonocytic
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CD33 protein, human
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Immunoglobulin G
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Immunotoxins
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Organic Chemicals
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Sialic Acid Binding Ig-like Lectin 3
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Gemtuzumab