Double-label analyses demonstrating the non-coexistence of enkephalin and glycine in amacrine cells of the larval tiger salamander retina

C B Watt; V J Florack

doi:10.1016/0006-8993(91)91200-k

Double-label analyses demonstrating the non-coexistence of enkephalin and glycine in amacrine cells of the larval tiger salamander retina

Brain Res. 1991 Oct 18;562(1):154-8. doi: 10.1016/0006-8993(91)91200-k.

Authors

C B Watt¹, V J Florack

Affiliation

¹ Alice R. McPherson Laboratory of Retina Research, Center for Biotechnology, Baylor College of Medicine, The Woodlands, TX 77381.

PMID: 1799867
DOI: 10.1016/0006-8993(91)91200-k

Abstract

Enkephalin immunocytochemistry was combined with either glycine immunocytochemistry or autoradiography of high-affinity glycine uptake to examine for colocalization of enkephalin and glycine in amacrine cells of the larval tiger salamander retina. A total of 995 enkephalin-immunoreactive amacrine cells were visualized in double-label preparations. None of the enkephalin-labelled cells was observed to co-label for markers of glycinergic activity.

Publication types

Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, P.H.S.

MeSH terms

Animals
Autoradiography
Caudata
Enkephalins / analysis*
Fluorescent Antibody Technique
Glycine / analysis*
Glycine / metabolism
Immunohistochemistry
Larva
Retina / cytology*
Tritium

Substances

Enkephalins
Tritium
Glycine

Grants and funding

EY05622/EY/NEI NIH HHS/United States