Abstract
In the present study, DNA sequencing of the genes SRGN, ARF6, AP3B1, and SH2D1A was performed in a well defined cohort of 18 families with familial hemophagocytic lymphohistiocytosis (FHL). A heterozygous nucleotide change (C > T) in the 3'untranslated region of the SRGN gene and a monoallelic 3-base pair deletion (c.2409_2411delGAA) in exon 21 of the AP3B1 gene were observed in two different families. Additionally, two novel polymorphisms, one in intron 17 of AP3B1 and another in intron 2 of SH2D1A were identified. We conclude that mutations in SRGN, ARF6, and AP3B1 are not likely to be common in patients fulfilling the FHL criteria.
(c) 2008 Wiley-Liss, Inc.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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3' Untranslated Regions / genetics
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ADP-Ribosylation Factor 6
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ADP-Ribosylation Factors / genetics*
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Adaptor Protein Complex 3 / genetics*
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Adaptor Protein Complex beta Subunits / genetics*
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Child
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Child, Preschool
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Cohort Studies
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DNA Mutational Analysis
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Exons / genetics
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Female
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Humans
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Infant
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Intracellular Signaling Peptides and Proteins / genetics*
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Lymphohistiocytosis, Hemophagocytic / genetics*
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Male
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Mutation / genetics
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Polymorphism, Genetic / genetics*
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Proteoglycans / genetics*
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Signaling Lymphocytic Activation Molecule Associated Protein
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Vesicular Transport Proteins / genetics*
Substances
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3' Untranslated Regions
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ADP-Ribosylation Factor 6
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AP3B1 protein, human
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Adaptor Protein Complex 3
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Adaptor Protein Complex beta Subunits
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Intracellular Signaling Peptides and Proteins
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Proteoglycans
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SH2D1A protein, human
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Signaling Lymphocytic Activation Molecule Associated Protein
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Vesicular Transport Proteins
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serglycin
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ADP-Ribosylation Factors
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ARF6 protein, human