Diffusion MRI is a technique that can probe diffusivity of water molecules in brain structure non-invasively and so that it presents complex neuronal orientation by discrete ribbons. In the study, a new tractography algorithm for high angular resolution diffusion imaging was developed to connect the discrete neural orientations and to extract sophisticated neuronal connectivity in human brain. By using volume of interesting selection interface and interaction of multiple regions by Boolean function, the results showed its potential of this technique in revealing complex neuronal pathways which could not be acquired from conventional techniques.