Diagnostic cellular organization features extracted from autofluorescence images

Opt Lett. 2007 Nov 15;32(22):3305-7. doi: 10.1364/ol.32.003305.

Abstract

Depth-resolved NADH autofluorescence images are shown to differentiate between normal and precancerous engineered tissues. An inverse power law behavior of the power spectral density (PSD) of these images is observed, indicating a self-affine organization of mitochondrial NADH at length scales 1-10 microm. Power exponents of the PSD functions vary significantly with tissue depth and precancerous state, giving insight into the morphological changes associated with precancerous lesions and providing substantial potential for noninvasive clinical diagnosis of squamous epithelial lesions and tumors.

MeSH terms

  • Fractals
  • Humans
  • Image Processing, Computer-Assisted
  • Laryngeal Neoplasms / metabolism
  • Laryngeal Neoplasms / pathology*
  • Membrane Microdomains / pathology
  • Microscopy, Fluorescence / instrumentation*
  • Microscopy, Fluorescence / methods*
  • Mitochondria / pathology*
  • NAD / chemistry*
  • Neoplasms / pathology*
  • Neoplasms, Squamous Cell / pathology*
  • Optics and Photonics*
  • Precancerous Conditions / metabolism
  • Precancerous Conditions / pathology*
  • Spectrometry, Fluorescence / instrumentation
  • Spectrometry, Fluorescence / methods

Substances

  • NAD