SAGA and a novel Drosophila export complex anchor efficient transcription and mRNA export to NPC

Maria M Kurshakova; Alexey N Krasnov; Daria V Kopytova; Yulii V Shidlovskii; Julia V Nikolenko; Elena N Nabirochkina; Danièle Spehner; Patrick Schultz; Làszlò Tora; Sofia G Georgieva

doi:10.1038/sj.emboj.7601901

SAGA and a novel Drosophila export complex anchor efficient transcription and mRNA export to NPC

EMBO J. 2007 Dec 12;26(24):4956-65. doi: 10.1038/sj.emboj.7601901. Epub 2007 Nov 22.

Authors

Maria M Kurshakova¹, Alexey N Krasnov, Daria V Kopytova, Yulii V Shidlovskii, Julia V Nikolenko, Elena N Nabirochkina, Danièle Spehner, Patrick Schultz, Làszlò Tora, Sofia G Georgieva

Affiliation

¹ Department of Regulation of Gene Expression, Institute of Gene Biology, Russian Academy of Sciences, Moscow, Russia.

Abstract

SAGA/TFTC-type multiprotein complexes play important roles in the regulation of transcription. We have investigated the importance of the nuclear positioning of a gene, its transcription and the consequent export of the nascent mRNA. We show that E(y)2 is a subunit of the SAGA/TFTC-type histone acetyl transferase complex in Drosophila and that E(y)2 concentrates at the nuclear periphery. We demonstrate an interaction between E(y)2 and the nuclear pore complex (NPC) and show that SAGA/TFTC also contacts the NPC at the nuclear periphery. E(y)2 forms also a complex with X-linked male sterile 2 (Xmas-2) to regulate mRNA transport both in normal conditions and after heat shock. Importantly, E(y)2 and Xmas-2 knockdown decreases the contact between the heat-shock protein 70 (hsp70) gene loci and the nuclear envelope before and after activation and interferes with transcription. Thus, E(y)2 and Xmas-2 together with SAGA/TFTC function in the anchoring of a subset of transcription sites to the NPCs to achieve efficient transcription and mRNA export.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Cell Line
Cell Nucleus / metabolism
Chromosomes / metabolism
Cryoelectron Microscopy
Drosophila Proteins / genetics
Drosophila Proteins / metabolism*
Drosophila Proteins / ultrastructure
Drosophila melanogaster / genetics
Drosophila melanogaster / metabolism*
Gene Expression Regulation
HSP70 Heat-Shock Proteins / genetics
HSP70 Heat-Shock Proteins / metabolism
Humans
Immunohistochemistry
Male
Multiprotein Complexes / chemistry
Multiprotein Complexes / metabolism*
Nuclear Envelope / metabolism
Nuclear Pore / metabolism*
Protein Subunits / genetics
Protein Subunits / metabolism
RNA Interference
RNA Transport / physiology*
RNA, Messenger / metabolism*
RNA-Binding Proteins / genetics
RNA-Binding Proteins / metabolism
Transcription Factors / genetics
Transcription Factors / metabolism*
Transcription Factors / ultrastructure
Transcription, Genetic*
Two-Hybrid System Techniques

Substances

Drosophila Proteins
HSP70 Heat-Shock Proteins
Multiprotein Complexes
Protein Subunits
RNA, Messenger
RNA-Binding Proteins
Transcription Factors
e(y)2 protein, Drosophila
xmas protein, Drosophila